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FITC anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1
LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
  • 16-10A1
    LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
Compare all formats See FITC spectral data
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104705 50 µg 57€
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104706 500 µg 164€
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  2. Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed
  3. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  4. Kis-Toth K, et al. 2011. J Immunol. 187:1273. PubMed
  5. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  6. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  7. Gravano D, et al. 2011. PLoS One. 6:e13517. PubMed
  8. Liang F, et al. 2021. RSC Adv. 11:35331. PubMed
  9. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  10. Saito S, et al. 2020. Nutrients. 12:. PubMed
  11. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  12. Porciello N, et al. 2018. Nat Commun. 9:1080. PubMed
  13. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  14. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  15. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  16. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  17. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  18. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  19. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  20. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  21. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  22. Jiang H, et al. 2018. Front Pharmacol. 9:612. PubMed
  23. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  24. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  25. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
  26. Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed
  27. Balashov L 2015. Mult Scler. 21: 1131-1138. PubMed
  28. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  29. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  30. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  31. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  32. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  33. Zhou Y, et al. 2020. Front Pharmacol. 10:1579. PubMed
  34. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  35. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  36. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  37. Li X, et al. 2009. PLoS One. 4:e6385. PubMed
  38. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  39. Ji W, et al. 2018. Evid Based Complement Alternat Med. 2017:3272649. PubMed
  40. Xiong W, et al. 2022. Adv Sci (Weinh). 9:e2103029. PubMed
  41. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  42. Gómez LA, et al. 2020. Front Microbiol. 11:1586. PubMed
  43. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  44. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  45. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  46. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  47. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  48. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  49. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  50. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  51. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  52. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  53. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  54. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  55. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  56. Ren S, et al. 2022. Int J Biol Sci. 18:166. PubMed
  57. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  58. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  59. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  60. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  61. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  62. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  63. Funk KE, et al. 2021. Aging Cell. 20:e13412. PubMed
  64. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  65. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  66. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
RRID
AB_313126 (BioLegend Cat. No. 104705)
AB_313127 (BioLegend Cat. No. 104706)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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