FITC anti-human/mouse Cutaneous Lymphocyte Antigen (CLA) Antibody

Pricing & Availability
HECA-452 (See other available formats)
Regulatory Status
V S075
Other Names
Cutaneous Lymphocyte-associated Antigen (CLA)
Rat IgM, κ
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Product Citations
Human peripheral blood lymphocytes stained with HECA-452 FITC
  • HECA-452_FITC_072407
    Human peripheral blood lymphocytes stained with HECA-452 FITC
Compare all formats See FITC spectral data
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321305 25 tests 83€
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321306 100 tests 187€
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Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References
  1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
  2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
  3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
  4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
  5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed
Product Citations
  1. Wan X, et al. 2013. Glycobiology. 23:1184. PubMed
  2. Thorleifsdottir R, et al. 2012. J Immunol. 188:5160. PubMed
  3. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  4. Nahar dos Santos L, et al. 2016. PLoS One. 11:e0164543. PubMed
  5. Port JR, et al. 2020. J Virol. 94:. PubMed
  6. Liu Z, et al. 2016. Sci Rep. 6:34416. PubMed
  7. Scottà C, et al. 2016. Haematologica. 101: 91 - 100. PubMed
  8. Zhao Y, et al. 2012. Rheumatology. 1.297222222. PubMed
  9. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
AB_492899 (BioLegend Cat. No. 321305)
AB_492898 (BioLegend Cat. No. 321306)

Antigen Details

140 kD, carbohydrate epitope of PSGL-1

T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes

Memory cells tethering and rolling
E-selectin, P-selectin, L-selectin
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.

Gene ID
6404 View all products for this Gene ID
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Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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