FITC anti-6-His Epitope Tag Antibody (Previously Covance catalog# FITC-156L)

Pricing & Availability
Clone
6-His (See other available formats)
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously
Covance Catalog# FITC-156L
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
906105 100 µL 286€
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
6xHis Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB, IP, ICC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000
IF: 1:1,000
IP: 1:150

Excitation Laser
Blue Laser (488 nm)
Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem. 276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations
  1. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID
AB_2565059 (BioLegend Cat. No. 906105)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/12/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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