Direct-Blot™ HRP anti-TCF1 (TCF7) Antibody

Pricing & Availability
Clone
7F11A10 (See other available formats)
Other Names
T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
7F11A10_DB_HRP_TCF1_Antibody_1_060118
Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.025, 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 7F11A10_DB_HRP_TCF1_Antibody_1_060118
    Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.025, 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 7F11A10_DB_HRP_TCF1_Antibody_2_060118
    Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.5, 1.5 and 2 µg/ml Direct-Blot™ HRP, or 2 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Quantity Avail. Save
655209 25 µL 68€
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655210 100 µL 232€
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Description

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial TCF1 recombinant protein (116-334 aa)
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:250-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734521 (BioLegend Cat. No. 655209)
AB_2734522 (BioLegend Cat. No. 655210)

Antigen Details

Structure
384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution

Nucleus

Function
TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction
TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
TCRs, Nuclear Markers
Antigen References

1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317.

Gene ID
6932 View all products for this Gene ID
UniProt
View information about TCF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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