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Brilliant Violet 785™ anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
29F.1A12 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, Programmed Death-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
29F.1A12_BV785_CD279_Antibody_FC_061614.jpg
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were stained with CD3 (clone 145-2C11) FITC and CD279 (clone 29F.1A12) Brilliant Violet 785™ (top), or rat IgG2a, κ Brilliant Violet 785™ isotype control (bottom).
  • 29F.1A12_BV785_CD279_Antibody_FC_061614.jpg
    Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were stained with CD3 (clone 145-2C11) FITC and CD279 (clone 29F.1A12) Brilliant Violet 785™ (top), or rat IgG2a, κ Brilliant Violet 785™ isotype control (bottom).
  • 29F.1A12_BV785_CD279_Antibody_FC_2_061614.jpg
Compare all formats See Brilliant Violet 785™ spectral data
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135225 50 µg 234€
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Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Application References
  1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
  3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
  4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  4. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  5. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  6. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  7. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  8. Ambler R, et al. 2020. Science Signaling. 13(649):. PubMed
  9. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  10. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  11. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  12. Wagle MV, et al. 2021. Nat Commun. 12:2782. PubMed
  13. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  14. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  15. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  16. Goc J, et al. 2021. Cell. 184:5015. PubMed
  17. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
RRID
AB_2563680 (BioLegend Cat. No. 135225)

Antigen Details

Structure
A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function
Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor
B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, T cells
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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