Brilliant Violet 510™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
30F11_BV510_CD45_Antibody_FC_083112
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 510™.
  • 30F11_BV510_CD45_Antibody_FC_083112
    C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 510™.
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability Save
103137 125 µL 146€
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103138 50 µg 208€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  2. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  3. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  4. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  5. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  6. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  7. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  8. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  9. Jacobson A et al. 2018. Cell host & microbe. 24(2):296-307 . PubMed
  10. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  11. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  12. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  13. Weinstock A, et al. 2019. Immunometabolism. 1. PubMed
  14. Xiao Y, et al. 2018. Int Immunol. 30:457. PubMed
  15. Duc D et al. 2019. Cell Rep. 29(2):378-390 . PubMed
  16. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  17. Thanabalasuriar A et al. 2019. Cell Host Microbe. 25(4):526-536 . PubMed
  18. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
  19. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  20. Silva DA, et al. 2019. Nature. 565:186. PubMed
  21. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  22. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  23. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  24. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  25. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  26. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  27. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  28. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  29. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  30. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  31. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  32. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  33. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  34. Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed
  35. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  36. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  37. Taguchi K, et al. 2015. J Urol. . PubMed
  38. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  39. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  40. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  41. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  42. Tian Y, et al. 2020. JCI Insight. 5:00. PubMed
  43. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  44. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  45. Li JY, et al. 2020. J Clin Invest. 130:1767. PubMed
  46. Benitez AA, et al. 2020. Commun Biol. 0.371527778. PubMed
  47. Cornax I, et al. 2021. Microorganisms. 9:00. PubMed
  48. Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed
  49. Tyagi AM, et al. 2021. eLife. 10:00. PubMed
  50. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  51. LaMarche NM, et al. 2020. Cell Metabolism. 32(2):243-258.e6. PubMed
  52. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  53. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  54. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  55. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  56. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  57. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  58. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  59. Xia L, et al. 2021. Cell Chemical Biology. 28(5):610-624.e5. PubMed
  60. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
  61. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  62. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  63. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  64. Dong L, et al. 2021. Cancer Cell. . PubMed
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RRID
AB_2561392 (BioLegend Cat. No. 103137)
AB_2563061 (BioLegend Cat. No. 103138)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC, IHC-F, CyTOF®, IP, CMCD, IHC, WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC, SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC, ICC, IHC, 3D IHC, SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC, SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F, FC, 3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC, SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC, CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC, CyTOF®, IP, CMCD, IHC, WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD45

  • Biotin anti-mouse CD45

  • FITC anti-mouse CD45

  • PE anti-mouse CD45

  • PE/Cyanine5 anti-mouse CD45

  • Purified anti-mouse CD45

  • PE/Cyanine7 anti-mouse CD45

  • APC/Cyanine7 anti-mouse CD45

  • Alexa Fluor® 488 anti-mouse CD45

  • Alexa Fluor® 647 anti-mouse CD45

  • Pacific Blue™ anti-mouse CD45

  • Alexa Fluor® 700 anti-mouse CD45

  • PerCP/Cyanine5.5 anti-mouse CD45

  • PerCP anti-mouse CD45

  • Alexa Fluor® 594 anti-mouse CD45

  • Brilliant Violet 421™ anti-mouse CD45

  • Brilliant Violet 570™ anti-mouse CD45

  • Brilliant Violet 510™ anti-mouse CD45

  • Brilliant Violet 605™ anti-mouse CD45

  • Purified anti-mouse CD45 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD45

  • Brilliant Violet 711™ anti-mouse CD45

  • Brilliant Violet 785™ anti-mouse CD45

  • Brilliant Violet 650™ anti-mouse CD45

  • APC/Fire™ 750 anti-mouse CD45

  • Brilliant Violet 750™ anti-mouse CD45

  • TotalSeq™-A0096 anti-mouse CD45

  • TotalSeq™-B0096 anti-mouse CD45

  • Ultra-LEAF™ Purified anti-mouse CD45

  • Spark Blue™ 550 anti-mouse CD45

  • Spark NIR™ 685 anti-mouse CD45

  • TotalSeq™-C0096 anti-mouse CD45

  • Spark YG™ 570 anti-mouse CD45

  • PE/Fire™ 640 anti-mouse CD45

  • APC/Fire™ 810 anti-mouse CD45

  • PE/Fire™ 700 anti-mouse CD45

  • Spark Violet™ 538 anti-mouse CD45

  • Spark YG™ 593 anti-mouse CD45

  • Spark Blue™ 574 anti-mouse CD45 Antibody

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