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Brilliant Violet 510™ anti-human TCR Vα24-Jα18 (iNKT cell) Antibody

Pricing & Availability
Clone
6B11 (See other available formats)
Regulatory Status
RUO
Other Names
TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B11_BV510_TCRVa24-Ja18_Antibody_FC_1_071614.jpg
Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα24-Jα18 (clone 6B11) Brilliant Violet 510™ (top) or mouse IgG1, κ Brilliant Violet 510™ isotype control (bottom).
  • 6B11_BV510_TCRVa24-Ja18_Antibody_FC_1_071614.jpg
    Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα24-Jα18 (clone 6B11) Brilliant Violet 510™ (top) or mouse IgG1, κ Brilliant Violet 510™ isotype control (bottom).
  • 6B11_BV510_TCRVa24-Ja18_Antibody_FC_2_071614.jpg
Compare all formats See Brilliant Violet 510™ spectral data
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342917 25 tests 155€
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342918 100 tests 331€
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Description

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.

Application References
  1. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
RRID
AB_2564005 (BioLegend Cat. No. 342917)
AB_2564006 (BioLegend Cat. No. 342918)

Antigen Details

Structure
Invariant CDR3 region of TCR Vα24-JαQ
Distribution

Invariant NKT cells

Ligand/Receptor
Glycolipid loaded CD1d
Cell Type
NKT cells
Biology Area
Immunology
Molecular Family
TCRs
Antigen References
  1. Thomas SY, et al. 2003. J. Immunol. 171:2571.
  2. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
  3. Montoya CJ, et al. 2007. Immunology. 122:1.
  4. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID
28659 View all products for this Gene ID
UniProt
View information about TCR Valpha24-Jalpha18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/16/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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