Brilliant Violet 510™ anti-human CD155 (PVR) Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

SK-N-S1 human neuroblastoma cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications include: block binding of PVR ligands CD226 and CD96 to PVR, immunoprecipitation, and western blot.

Application References

1. Fuchs A, et al. 2004. J. Immunol. 172:3994

Product Citations

1. Braun DA, et al. 2021. Cancer Cell. 39:632. PubMed

RRID

AB_2810527 (BioLegend Cat. No. 337633)
AB_2810528 (BioLegend Cat. No. 337634)

Antigen Details

Structure

Type I glycoprotein, Ig superfamily

Distribution

Monocytes, endothelial cells, epithelia, central nervous system

Function

Cell-cell and cell-matrix adhesion

Ligand/Receptor

CD226, CD96, nectin-1, -2, and -3, vitronectin, CD155, poliovirus

Cell Type

Endothelial cells, Epithelial cells, Monocytes, Tregs

Biology Area

Immunology, Innate Immunity

Molecular Family

Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication
2. Bottino PD, et al. 2005. Mol. Immunol. 42:463
3. Tahara-Hanaoka S, et al. 2004. Intl. Immunol. 16:533

Gene ID

5817 View all products for this Gene ID

UniProt

View information about CD155 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis