Brilliant Violet 421™ anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
4B10_BV421_T-Bet_Antibody_ICFC_1_021715
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4B10_BV421_T-Bet_Antibody_ICFC_1_021715
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4B10_BV421_T-Bet_Antibody_ICFC_2_021715
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
644815 125 µl 156€
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644832 50 µg 208€
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644816 500 µl 292€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
µl size: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
µg size: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Aalderen M, et al. 2013. J Virol. 87:10263. PubMed
  2. Schaik B, et al. 2015. J Virol. 89:568. PubMed
  3. Aalderen M, et al. 2015. J Virol. 89:5110. PubMed
  4. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  5. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  6. Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed
  7. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  8. Lorentsen KJ, et al. 2018. Nat Commun. 9:1679. PubMed
  9. Hirota K 2018. Immunity. 48:1220. PubMed
  10. Linehan JL 2018. Cell. 172:784. PubMed
  11. Han SJ, et al. 2017. Immunity. 47:1154. PubMed
Publication Library
RRID
AB_10896427 (BioLegend Cat. No. 644815)
AB_2686976 (BioLegend Cat. No. 644832)
AB_10959653 (BioLegend Cat. No. 644816)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 4    Revision Date: 02/17/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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