- A20 (See other available formats)
- Regulatory Status
- Other Names
- T200, Ly-5.1, LCA
- Mouse (A.SW) IgG2a, κ
- Ave. Rating
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- Product Citations
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CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.Product Details
- Antibody Type
- Host Species
- SJL mouse thymocytes and splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (please contact technical support for concentration and total µg amount)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunofluorescent staining using the µL size, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
- Application References
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
- Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
- Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
- Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
- Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
- Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
- Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
- Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
- Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
- Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
- Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
- Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
- Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
- Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
AB_10896425 (BioLegend Cat. No. 110731)
AB_2562563 (BioLegend Cat. No. 110732)
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
- Phosphatase, T and B cell activation
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- View information about CD45.1 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
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