- B-D48 (See other available formats)
- Regulatory Status
- Other Names
- PRF1, P1, PFP, HPLH2
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
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Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Product Citations
AB_11149688 (BioLegend Cat. No. 353307)
- 70 kD
CTL, NK (cytoplasmic granules)
- Mediates targeted cell lysis.
- Cell Type
- T cells, NK cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Antigen References
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
- Gene ID
- 5551 View all products for this Gene ID
- View information about Perforin on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
|Brilliant Violet 421™ anti-human Perforin||B-D48||ICFC|
|PE anti-human Perforin||B-D48||ICFC|
|Pacific Blue™ anti-human Perforin||B-D48||ICFC|
|APC anti-human Perforin||B-D48||ICFC|
|PerCP/Cyanine5.5 anti-human Perforin||B-D48||ICFC|
|PE/Cyanine7 anti-human Perforin||B-D48||ICFC|
|FITC anti-human Perforin||B-D48||ICFC|
|Alexa Fluor® 700 anti-human Perforin||B-D48||ICFC|
|APC/Fire™ 750 anti-human Perforin||B-D48||ICFC|
|Alexa Fluor® 488 anti-human Perforin||B-D48||ICFC|
|Alexa Fluor® 647 anti-human Perforin||B-D48||ICFC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Brilliant Violet 421™ anti-human Perforin
PE anti-human Perforin
Pacific Blue™ anti-human Perforin
APC anti-human Perforin
PerCP/Cyanine5.5 anti-human Perforin
PE/Cyanine7 anti-human Perforin
FITC anti-human Perforin
Alexa Fluor® 700 anti-human Perforin
APC/Fire™ 750 anti-human Perforin
Alexa Fluor® 488 anti-human Perforin
Alexa Fluor® 647 anti-human Perforin