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Brilliant Violet 421™ anti-human MERTK Antibody

Pricing & Availability
Clone
590H11G1E3 (See other available formats)
Regulatory Status
RUO
Other Names
C-Mer Proto-Oncogene Tyrosine Kinase, c-mer, Proto-Oncogene C-Mer, MER Receptor Tyrosine Kinase, STK Kinase, Receptor Tyrosine Kinase MerTK, Tyrosine-Protein Kinase Mer, MER, RP38, c-Eyk, Tyro12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
590H11G1E3_BV421_MERTK_Antibody_FC_092315
Human peripheral blood monocytes were stimulated and cultured with M-CSF for seven days then stained with human MERTK (clone 590H11G1E3) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 590H11G1E3_BV421_MERTK_Antibody_FC_092315
    Human peripheral blood monocytes were stimulated and cultured with M-CSF for seven days then stained with human MERTK (clone 590H11G1E3) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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367603 25 tests 164€
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367604 100 tests 348€
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Description

MERTK plays a role in the retinal pigment epithelium as a regulator of rod outer segments fragments phagocytosis. MERTK also plays a role in the inhibition of Toll-like receptor-mediated innate immune responses through the activation of STAT1. Upregulation of MERTK seems to also promote the survival of certain cancer cells, such as t(1;19)-positive acute lymphoblastic leukemias (ALL). MERTK also has a role in cellular migration, as MERTK KO macrophages demonstrate cytoskeletal disruptions that impacts its shape and directional migration. Melanoma cells express high levels of MERTK, which makes this molecule an attractive therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MERTK extracellular domain/Fc fusion.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
  1. Rogers AE, et al. 2012. Oncogene 31:4171.
Product Citations
  1. Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
  2. Zheng X, et al. 2020. Cancer Res. 4414:80. PubMed
  3. Holtzhausen A, et al. 2019. Cancer Immunol Res. 7:1672. PubMed
RRID
AB_2566396 (BioLegend Cat. No. 367603)
AB_2566397 (BioLegend Cat. No. 367604)

Antigen Details

Structure
Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.
Distribution

Macrophages, monocytes, and dendritic cells.

Function
TLR regulation, efferocytosis, phagocytosis regulation, cell survival, and macrophage migration.
Interaction
GRB2, PLCG2, and TNK2.
Ligand/Receptor
LGALS3, TUB, TULP1, and GAS6.
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Protein Kinases/Phosphatase, Toll Like Receptors
Antigen References

1. Schlegel J, et al. 2013. J. Clin. Invest. 123:2257.
2. Chen J, et al. 1997. Oncogene 14:2033.
3. Yefimova MG, et al. 2013. Autophagy 9:653.
4. Zhang W, et al. 2013. J. Med. Chem. 56:9693.
5. Lee YJ, et al. 2012. J. Leuko. Biol. 91:921.
6. Krause S, et al. 2015. Blood 125:820.
7. Tang Y, et al. 2015. PLoS One 10:e0117787.

Gene ID
10461 View all products for this Gene ID
UniProt
View information about MERTK on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 09/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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