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Biotin anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_Biotin_082808.jpg
C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
  • 1A8_Biotin_082808.jpg
    C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
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127603 50 µg 62€
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127604 500 µg 216€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed
  3. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  4. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  5. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  6. Blazek K, et al. 2015. J Exp Med. 212:845. PubMed
  7. Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed
  8. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  9. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  10. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  11. Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed
  12. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  13. Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed
  14. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  15. Lerman I, et al. 2019. Mol Cancer Res. 17:845. PubMed
  16. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  17. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  18. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  19. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  20. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  21. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  22. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  23. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  24. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  25. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  26. Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed
  27. Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed
  28. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  29. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  30. Chen Z, et al. 2021. Front Immunol. 12:641874. PubMed
  31. Muthukrishnan G, et al. 2021. Frontiers in Immunology. 12:651515. PubMed
  32. Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed
  33. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  34. Yang L, et al. 2012. J Immunol. 189:3741. PubMed
  35. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  36. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  37. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  38. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  39. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  40. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  41. Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
  42. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  43. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  44. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  45. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  46. Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed
  47. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  48. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  49. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  50. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  51. Morita Y, et al. 2022. Bone Res. 10:56. PubMed
  52. Huang X, et al. 2021. Immunity. . PubMed
  53. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  54. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
  55. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  56. De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed
  57. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
  58. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
RRID
AB_1186105 (BioLegend Cat. No. 127603)
AB_1186108 (BioLegend Cat. No. 127604)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Go To Top Version: 3    Revision Date: 01/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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