- MP2-43D11 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
- Rat IgG2a, κ
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- Product Citations
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IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-43D11 antibody reacts with mouse interleukin-3 (IL-3). The MP2-43D11 antibody can not neutralize the bioactivity of natural or recombinant IL-3.Product Details
- Antibody Type
- Host Species
- COS-expressed, recombinant mouse IL-3
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate.
For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Detection1-5,7: The biotinylated MP2-43D11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP2-8F8 antibody (Cat. No. 503902/503906) as the capture antibody.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting, and immunohistochemical staining6 of paraformaldehyde-fixed, saponin-treated tissue sections.
- Application References
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Abrams, J., et al. 1988. J. Immunol. 140:131.
- Cockayne, D., et al. 1991. Growth Factors 5:171.
- Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
- Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Sander, B., et al. 1991. Immunol. Rev. 119:65.
- Karulin, A., et al. 2000. J. Immunol. 164:1862.
AB_2123875 (BioLegend Cat. No. 504002)
- Cytokine; 28 kD (Mammalian)
- Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
- Cell Sources
- Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
- Cell Targets
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
- Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.
- Upregulated by T cell stimulation; downregulated by glucocorticoids
- Gene ID
- 16187 View all products for this Gene ID
- View information about IL-3 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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