Biotin anti-human G-CSF Antibody

Pricing & Availability
Clone
BVD11-37G10 (See other available formats)
Other Names
Granulocyte-Colony Stimulating Factor, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)
Isotype
Rat IgG2a, κ
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Cat # Size Price Quantity Avail. Save
506702 500 µg 220€
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Description

Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. The BVD11-37G10 antibody reacts with human granulocyte-colony stimulating factor (G-CSF).The BVD11-37G10 antibody can neutralize the bioactivity of natural or recombinant G-CSF.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant human G-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1,2: The biotinylated BVD11-37G10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD13-3A5 antibody (Cat. No. 502102/502104) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, neutralization1,2, and immunohistochemistry3,4.

Application References
  1. Abrams J, et al. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  3. Andersson J, et al. 1994. Immunol. 83:16.
  4. Sander B, et al. 1991. Immunol. Rev. 119:65.
RRID
AB_2083731 (BioLegend Cat. No. 506702)

Antigen Details

Structure
Cytokine; 21 kD (Mammalian)
Bioactivity
Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
Cell Sources
Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
Cell Targets
Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
Receptors
CD114 (G-CSFR)
Cell Type
Neutrophils
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Moore M. 1991. Annu. Rev. Immunol. 9:159.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
Gene ID
1441 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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