ATPase Assay Kit

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Other Names
ATPase Assay, ATPase colorimetric assay
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ATPase_Assay_Kit_060916
Example of Pi Standard Curve.
  • ATPase_Assay_Kit_060916
    Example of Pi Standard Curve.
  • ATPase_Assay_Kit_Packaging_120116
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689001 480 tests 724€
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Description

The ATPase colorimetric assay kit employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PhosChrome™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising out of non-enzymatic ATP hydrolysis.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

PhosChrome™ - 1 bottle, 25mL
Accelerator - 1 vial, 0.5mL
Stabilizer - 1 bottle, 10mL
0.1M MgCl2 - 2 vials, 1.5mL
0.5M Tris pH 7.5 - 1 bottle, 10mL
0.1mM Pi Standard - 1 bottle, 10mL
ATP - 10 vials, lyophilized
96-well plates - 5 plates

Product Details

Application

Enzyme activity assay

Recommended Usage

See manual for instructions.

Application Notes

Key Features:

  • High throughput colorimetric enzymatic assay
  • Simple to use, scalable, and non-radioactive
  • Compatible with any assay buffer
  • Stable reagent formulation that ensures long shelf life
  • Suppresses non enzymatic backgrounds with acid-labile substrates
  • No precipitation, results can be measured over several hours
  • Wide linear range, no inhibition of color development by high concentrations of protein

 

How it works:

Assays are based on the formation of green colored complexes between an inorganic phosphate and PhosChrome™ (an orange colored superior malachite green reagent) under acidic conditions.

Application References
  1. Schuhmacher JS, et al. 2015. Molecular Microbiology 10:3092-3097.
  2. Zhang J, et al. 2015. PLoS Pathog. 11(3):e1004768.
  3. Fishburn J, et al. 2015. Proc. Natl. Acad. Sci. USA 112(13):3961.
  4. Robinson M, et al. 2012. The FASEB Journal 26(11):4614-27.
  5. Sarraf N, et al. 2014. PLoS One. 9:e100441.
  6. Xiao X, et al. 2013. PLoS One. 8:e83610.
  7. Matos M, et al. 2013. J. Neurosci. 33:18492-18502.
  8. Luo H, et al. 2013. Biomed Environ Sci. 26:231-242
  9. Bruchmann A, et al. 2013. BMC Cancer. 13: 96.
  10. Ronchi D, et al. 2013. Am. J. Hum. Genet. 92: 293-300.
  11. Choi CH, et al. 2013. Cell. Microbiol. 15:961-976.
  12. Podhajska A, et al. 2012. PLoS One. 7: e39942.

Antigen Details

Function
ATPase enzymes hydrolyze ATP into ADP and inorganic phosphate (Pi).
Biology Area
Cell Biology, Cell Proliferation and Viability
Gene ID
NA

Related FAQs

I have 5% DMSO in my assay. Can I use PhosChrome™?

Yes, the reagent is designed for drug screening work and other situations that require DMSO.

I have phosphate in my enzyme. What can I do?

You can dialyze or desalt the enzyme into a phosphate-free buffer.

Why do I get a high background when my enzyme definitely has no free Pi?

This is almost certainly caused by inadequate mixing of the Stabilizer. This results in a high background signal because of non-enzymatic decay of ATP substrate. The Stabilizer is added in a relatively small volume (20μL), and the operation of pipetting up and down with a pipette set to 20μL volume may not result in sufficient mixing when the total volume is 270μL. Try pipetting up and down while stirring at the same time. Alternatively, add the Stabilizer with one pipette set at 20μL volume and mix using a larger pipette set to ~150μL volume. This ensures thorough mixing of the Stabilizer solution with minimal effort.

Go To Top Version: 3    Revision Date: 12/28/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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