APC Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
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Product Citations
publications
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400511 25 µg 44€
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400512 100 µg 86€
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  2. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  3. Choi JG, et al. 2020. Front Immunol. 11:598556. PubMed
  4. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  5. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  6. Sullivan D, Watson R, Muller W 2016. Am J Physiol Heart Circ Physiol. 311: H621 - H632. PubMed
  7. Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed
  8. Acoba MG, et al. 2021. Cell Reports. 34(11):108869. PubMed
  9. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  10. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  12. McPherson HR, et al. 2021. Elife. 10:. PubMed
  13. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  14. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  15. Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed
  16. Mobarak E, et al. 2018. Sci Rep. 8:13600. PubMed
  17. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  18. Jiao Q, et al. 2020. Front Immunol. 11:469. PubMed
  19. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  20. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  21. Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
  22. Li Y, et al. 2014. Cell Death Dis. 5:1550. PubMed
  23. Thinard R, et al. 2022. Pharmaceutics. 14:. PubMed
  24. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  25. Berens E, et al. 2016. Oncogene. 10.1038/onc.2016.234. PubMed
  26. Abraham V, et al. 2018. Int J Oncol. 53:488. PubMed
  27. Bainor AJ et al. 2018. Cell reports. 25(10):2797-2807 . PubMed
  28. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  29. Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23: . PubMed
  30. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  31. Hogarth MW, et al. 2019. Nat Commun. 10:2430. PubMed
  32. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  33. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  34. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  35. Parker K, et al. 2014. Cancer Res. 74:5723. PubMed
  36. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  37. Yang T, et al. 2022. Antioxidants (Basel). 11:. PubMed
  38. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  39. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  40. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  41. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  42. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  43. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  44. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  45. Zhang T, et al. 2021. Journal of Neurochemistry. 157(3):611-623. PubMed
  46. He B, et al. 2018. Nat Commun. 9:5240. PubMed
  47. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  48. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  49. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  50. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  51. Moudgil A, et al. 2020. Cell. 182(4):992-1008.e21. PubMed
  52. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  53. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
RRID
AB_2814702 (BioLegend Cat. No. 400512)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl RTK2758 SB
PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

    RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...
  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl

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