- Clone
- A019D5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-7 receptor α chain, IL-7Rα
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD127 (IL-7Rα) (clone A019D5) APC/Fire™ 810 (left) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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351373 | 25 tests | 156€ | ||||
351374 | 100 tests | 366€ |
CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CD127
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported (for the relevant formats) application: proteogenomics1.
- Application References
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- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
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AB_2904371 (BioLegend Cat. No. 351373)
AB_2904371 (BioLegend Cat. No. 351374)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701. - Gene ID
- 3575 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
Related FAQs
Other Formats
View All CD127 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD127 (IL-7Rα)
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PE anti-human CD127 (IL-7Rα)
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Pacific Blue™ anti-human CD127 (IL-7Rα)
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Brilliant Violet 421™ anti-human CD127 (IL-7Rα)
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FITC anti-human CD127 (IL-7Rα)
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Alexa Fluor® 488 anti-human CD127 (IL-7Rα)
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APC anti-human CD127 (IL-7Rα)
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Alexa Fluor® 647 anti-human CD127 (IL-7Rα)
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PE/Cyanine7 anti-human CD127 (IL-7Rα)
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PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)
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Brilliant Violet 570™ anti-human CD127 (IL-7Rα)
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PE/Cyanine5 anti-human CD127 (IL-7Rα)
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Brilliant Violet 650™ anti-human CD127 (IL-7Rα)
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Brilliant Violet 711™ anti-human CD127 (IL-7Rα)
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Brilliant Violet 785™ anti-human CD127 (IL-7Rα)
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Brilliant Violet 510™ anti-human CD127 (IL-7Rα)
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Brilliant Violet 605™ anti-human CD127 (IL-7Rα)
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PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)
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Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)
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Alexa Fluor® 700 anti-human CD127 (IL-7Rα)
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Biotin anti-human CD127 (IL-7Rα)
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APC/Cyanine7 anti-human CD127 (IL-7Rα)
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APC/Fire™ 750 anti-human CD127 (IL-7Rα)
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TotalSeq™-A0390 anti-human CD127 (IL-7Rα)
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TotalSeq™-B0390 anti-human CD127 (IL-7Rα)
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TotalSeq™-C0390 anti-human CD127 (IL-7Rα)
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KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)
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Spark NIR™ 685 anti-human CD127 (IL-7Rα)
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PE/Fire™ 640 anti-human CD127 (IL-7Rα)
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PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody
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Spark YG™ 581 anti-human CD127 (IL-7Rα)
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Brilliant Violet 750™ anti-human CD127 (IL-7Rα)
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TotalSeq™-D0390 anti-human CD127 (IL-7Rα)
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APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody
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APC/Fire™ 750 anti-human CD127
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PE anti-human CD127
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PerCP/Cyanine5.5 anti-human CD127
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PE/Cyanine7 anti-human CD127
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Spark Red™ 718 anti-human CD127 (IL-7Rα)
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