APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody, CD127, A019D5

Pricing & Availability

Clone: A019D5 (See other available formats)
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
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Product Citations: publications

A019D5_APCFire810_CD127_Antibody_102221 — Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD127 (IL-7Rα) (clone A019D5) APC/Fire™ 810 (left) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (right).

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351373

25 tests

156€

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351374

100 tests

366€

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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human CD127

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported (for the relevant formats) application: proteogenomics¹.

Application References

Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2904371 (BioLegend Cat. No. 351373)
AB_2904371 (BioLegend Cat. No. 351374)

Antigen Details

Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD127 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD127 (IL-7Rα)	A019D5	FC
PE anti-human CD127 (IL-7Rα)	A019D5	FC
Pacific Blue™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)	A019D5	FC
FITC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)	A019D5	FC
APC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)	A019D5	FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)	A019D5	FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα)	A019D5	FC
Biotin anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα)	A019D5	PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)	A019D5	FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
Spark YG™ 581 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα)	A019D5	PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
APC/Fire™ 750 anti-human CD127	A019D5	FC
PE anti-human CD127	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127	A019D5	FC
PE/Cyanine7 anti-human CD127	A019D5	FC
Spark Red™ 718 anti-human CD127 (IL-7Rα)	A019D5	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/22/2021

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