APC/Fire™ 750 Streptavidin

Pricing & Availability
Regulatory Status
Other Names
Streptavidin-Allophycocyanin, SAv-APC
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Product Citations
Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
  • Strepavidin_APCFIRE_Antibody_FC_110816
    Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
See APC/Fire™ 750 spectral data
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405250 100 µg 165€
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Streptavidin binds to biotin with high affinity. Streptavidin-APC/Fire™ 750 is useful for detecting biotinylated antibodies. The excitation of APC/Fire™ 750 by 600-635 nm laser light induces a fluorescence emission maximum of 787 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Streptavidin is conjugated with APC/Fire™ 750 under optimal conditions.
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Fire™ 750 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin APC/Fire™ 750 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Fire™ 750 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

*APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Streptavidin-Allophycocyanin/Fire™ 750 (APC/Fire™ 750 ) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Fire™ 750 is 350 kD and Streptavidin alone is 52 kD.

Application References
  1. Maresz K, et al. 2005. J. Neurochemistry 95:437.
  2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed.
  3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
  4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
  5. Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed
  6. Cao YA, et al. 2008. Blood PubMed
  7. Garidou L, et al. 2009. J. Virol. PubMed
  8. Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed
  9. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed.
  10. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
  11. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
  12. Prince J, et al. 2013. J. Immunol. 191:4709. PubMed
  13. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
  14. Orre M, et al. 2014. Neurbiol Aging. 35:1. PubMed
  15. Barbera M, et al. 2014. Gut. 64:11. PubMed
  16. Zhang Y, et al. 2014. Infect Immun. 82:1698. PubMed
  17. Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed
  18. Barbera M, et al. 2015. Gut. 64:11. PubMed
  19. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
Product Citations
  1. Mudd PA, et al. 2022. Cell. 185:603. PubMed

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