APC/Cyanine7 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_APCCyanine7_F4-80_Antibody_020119
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • BM8_APCCyanine7_F4-80_Antibody_020119
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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123117 25 µg 111€
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123118 100 µg 260€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Herbst S, et al. 2013. Dis Model Mech. 6:643. PubMed
  2. Ohta S, et al. 2013. J Immunol. 190:5927. PubMed
  3. Mason K, et al. 2013. J Immunol. 191:2457. PubMed
  4. Zordan P, et al. 2013. Eur J Pharmacol. 715:296. PubMed
  5. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  6. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  7. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  8. Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed
  9. Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed
  10. Taguchi K, et al. 2015. J Urol. . PubMed
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  13. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  14. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  15. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  16. DeFalco T, et al. 2016. Cell Rep. 12: 1107-1119. PubMed
  17. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  18. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
  19. Chen Z, et al. 2016. Innate Immunity. 22: 419 - 432. PubMed
  20. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  21. Chen C, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.02.040. PubMed
  22. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  23. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  24. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  25. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  26. Kahn JH, et al. 2020. Endocrinology. 161(10):. PubMed
  27. Vicario N, et al. 2021. Cell Death Disease. 12(7):625. PubMed
  28. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  29. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  30. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  31. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  32. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  33. Ushio A, et al. 2017. Int J Mol Sci. 10.3390/ijms18061209. PubMed
  34. Resende M, et al. 2017. J Immunol. 10.4049/jimmunol.1601701. PubMed
  35. Natale CA, et al. 2018. Elife. 7. PubMed
  36. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
  37. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  38. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  39. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  40. Celià-Terrassa T, et al. 2017. Nat Cell Biol. 19:711. PubMed
  41. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  42. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  43. Pilch Z, et al. 2019. PLoS One. 14:e0210796. PubMed
  44. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  45. Guo M, et al. 2018. J Immunol. 200:1039. PubMed
  46. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  47. Kozijn AE, et al. 2019. Osteoarthritis Cartilage. 27:118. PubMed
  48. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  49. Haloul M, et al. 2019. Sci Rep. 9:14050. PubMed
  50. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  51. Leiria LO et al. 2019. Cell Metab. 30(4):768-783 . PubMed
  52. Morrissey MA, et al. 2018. Elife. 7:e36688. PubMed
  53. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  54. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  55. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  56. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  57. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  58. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  59. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  60. Li J, et al. 2018. Immunity. 49:178. PubMed
  61. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
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RRID
AB_893489 (BioLegend Cat. No. 123117)
AB_893477 (BioLegend Cat. No. 123118)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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