APC anti-mouse LPAM-1 (Integrin α4β7) Antibody

Pricing & Availability
DATK32 (See other available formats)
Regulatory Status
Other Names
α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
Rat IgG2a, κ
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Product Citations
C57BL/6 splenocytes stained with DATK32 APC
  • DATK32_APC_082610
    C57BL/6 splenocytes stained with DATK32 APC
  • DATK32_APC_1_101310
    C57BL/6 mouse bone marrow cells were surface stained with B220 PE and LPAM-1 APC (clone DATK32) (top) or rat IgG2a, κ APC isotype control (bottom).
  • DATK32_APC_2_101310
See APC spectral data
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120607 25 µg 72,00€
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120608 100 µg 216,00€
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DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
TK1 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo

Application References
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185. (Block)
  3. Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block)
  4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP)
  5. Bogetto L, et al. 2000. Blood 95:2397. (IHC)
Product Citations
  1. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  2. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  3. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  4. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  5. Ghaedi M, et al. 2020. The Journal of Experimental Medicine. 217(3):jem. PubMed
  6. Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed
  7. Kawano H, et al. 2016. Int Immunol. 28: 489 - 501. PubMed
  8. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
AB_10719833 (BioLegend Cat. No. 120607)
AB_10730607 (BioLegend Cat. No. 120608)

Antigen Details

Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Lymphocyte adhesion
VCAM-1 (CD106), MAdCAM-1, fibronectin
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Berlin C, et al. 1994. Cell 74:185.
3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243.
4. Hamann A, et al. 1994. J. Immunol. 152:3282.

Gene ID
311144 View all products for this Gene ID 25713 View all products for this Gene ID
View information about LPAM-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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