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APC anti-mouse CD49b Antibody

Pricing & Availability
Clone
HMα2 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HMalpha2_APC_CD49b_Antibody_FC_1_071715
C57BL/6 splenocytes were stained with NK1.1 PE and CD49b (clone HMα2) APC (top), or Armenian Hamster IgG APC isotype control (bottom).
  • HMalpha2_APC_CD49b_Antibody_FC_1_071715
    C57BL/6 splenocytes were stained with NK1.1 PE and CD49b (clone HMα2) APC (top), or Armenian Hamster IgG APC isotype control (bottom).
  • HMalpha2_APC_CD49b_Antibody_FC_2_071715
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
103515 25 µg 82€
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103516 100 µg 234€
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Description

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse colon carcinoma cell line Colon26
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).

Application References
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
  4. Zhang Z, et al. 2008. Blood 111:1980. (IF)
Product Citations
  1. Xu X, et al. 2020. Chin Med. 15:33. PubMed
  2. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  3. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  4. Ye X, et al. 2021. Cancer Biol Med. Online ahead of print. PubMed
  5. Engeroff P, et al. 2020. J Allergy Clin Immunol. 145:301. PubMed
  6. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
RRID
AB_2566100 (BioLegend Cat. No. 103515)
AB_2566101 (BioLegend Cat. No. 103516)

Antigen Details

Structure
Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution

NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets

Function
Adhesion, lymphocyte activation
Ligand/Receptor
Collagen, laminin, fibronectin
Cell Type
Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/20/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD49b

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  • APC anti-mouse CD49b

  • PE/Cyanine7 anti-mouse CD49b

  • PerCP/Cyanine5.5 anti-mouse CD49b

  • Biotin anti-mouse CD49b

  • TotalSeq™-A0421 anti-mouse CD49b

  • TotalSeq™-C0421 anti-mouse CD49b

  • Ultra-LEAF™ Purified anti-mouse CD49b

  • TotalSeq™-B0421 anti-mouse CD49b

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