Alexa Fluor® 700 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_Alx700_080408.jpg
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
  • UCHT1_Alx700_080408.jpg
    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
300423 25 µg 84,00€
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300424 100 µg 180,00€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Product Citations
  1. Na I, et al. 2013. Haematologica. 98:23. PubMed
  2. Laprevotte E, et al. 2013. Leuk Res. 37:440. PubMed
  3. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  4. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  5. Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed
  6. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  7. Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed
  8. Kiniry B, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.100. PubMed
  9. Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed
  10. Hood SP, et al. 2020. Elife. 9:00. PubMed
  11. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  12. Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
  13. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  14. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  15. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  16. Keustermans G,et al. 2017. PLoS One. 10.1371/journal.pone.0187068. PubMed
  17. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  18. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  19. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
  20. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  21. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  22. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  23. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  24. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  25. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  26. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  27. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  28. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  29. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  30. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  31. Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed
  32. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  33. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  34. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  35. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
RRID
AB_493740 (BioLegend Cat. No. 300423)
AB_493741 (BioLegend Cat. No. 300424)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC, CyTOF®, IHC-F, IP, Activ, WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC, ICC, IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC, ICC, IHC-F
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC, CyTOF®, IHC-F, IP, Activ, WB
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC, FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC, CyTOF®
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC, ICC, IHC-F
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
Pacific Blue™ anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3

  • Biotin anti-human CD3

  • FITC anti-human CD3

  • PE anti-human CD3

  • PE/Cyanine5 anti-human CD3

  • Purified anti-human CD3

  • Alexa Fluor® 647 anti-human CD3

  • Alexa Fluor® 488 anti-human CD3

  • PerCP anti-human CD3

  • PerCP/Cyanine5.5 anti-human CD3

  • Ultra-LEAF™ Purified anti-human CD3

  • Alexa Fluor® 594 anti-human CD3

  • APC/Cyanine7 anti-human CD3

  • Brilliant Violet 570™ anti-human CD3

  • Purified anti-human CD3 (Maxpar® Ready)

  • Brilliant Violet 605™ anti-human CD3

  • Brilliant Violet 650™ anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • Brilliant Violet 421™ anti-human CD3

  • APC/Fire™ 750 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • Brilliant Violet 510™ anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • TotalSeq™-A0034 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • PE anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • KIRAVIA Blue 520™ anti-human CD3

  • Spark Blue™ 574 anti-human CD3 Antibody

  • TotalSeq™-B0034 anti-human CD3

  • TotalSeq™-C0034 anti-human CD3

  • TotalSeq™-D0034 anti-human CD3

  • Brilliant Violet 711™ anti-human CD3

  • Brilliant Violet 785™ anti-human CD3

  • Spark Violet™ 538 anti-human CD3 Antibody

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