Alexa Fluor® 647 anti-Tau, 368-441 Antibody

Pricing & Availability
Clone
A16097F (See other available formats)
Other Names
Microtubule associated protein tau
Isotype
Rat IgG2a, κ
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Product Citations
publications
A16097F_A647_Tau368-441_Antibody_030719
IHC staining of Alexa Fluor® 647 anti-Tau, 368-441 antibody (clone A16097F) on formalin-fixed paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.) (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 40 µm
  • A16097F_A647_Tau368-441_Antibody_030719
    IHC staining of Alexa Fluor® 647 anti-Tau, 368-441 antibody (clone A16097F) on formalin-fixed paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.) (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 40 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
851205 25 µg 108€
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851206 100 µg 220€
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 231-441.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody cross-reacts with human 3R and 4R Tau isoforms, and recognizes endogenous human Tau in brain lysates by WB. The antibody has minimal reactivity with mouse Tau protein.

RRID
AB_2801195 (BioLegend Cat. No. 851205)
AB_2801196 (BioLegend Cat. No. 851206)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
  3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 368-441 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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