- Clone
- 11-26c.2a (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin D
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647 -
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD68 Antibody (clone FA-11) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # | Size | Price | Quantity Check Availability | Save | ||
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405707 | 25 µg | 90€ | ||||
405708 | 100 µg | 212€ |
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
3D IHC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
- Application References
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- Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
- Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
- Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
- Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_893529 (BioLegend Cat. No. 405707)
AB_893528 (BioLegend Cat. No. 405708)
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Function
- B cell differentiation
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 380797 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse IgD
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PE anti-mouse IgD
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Purified anti-mouse IgD
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PerCP anti-mouse IgD
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Biotin anti-mouse IgD
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Brilliant Violet 711™ anti-mouse IgD
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Alexa Fluor® 700 anti-mouse IgD
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Alexa Fluor® 647 anti-mouse IgD
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PerCP/Cyanine5.5 anti-mouse IgD
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Pacific Blue™ anti-mouse IgD
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APC anti-mouse IgD
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APC/Cyanine7 anti-mouse IgD
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Alexa Fluor® 488 anti-mouse IgD
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PE/Cyanine7 anti-mouse IgD
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Brilliant Violet 650™ anti-mouse IgD
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Brilliant Violet 510™ anti-mouse IgD
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Brilliant Violet 421™ anti-mouse IgD
-
Brilliant Violet 605™ anti-mouse IgD
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Purified anti-mouse IgD (Maxpar® Ready)
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Alexa Fluor® 594 anti-mouse IgD
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PE/Dazzle™ 594 anti-mouse IgD
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APC/Fire™ 750 anti-mouse IgD
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TotalSeq™-A0571 anti-mouse IgD
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TotalSeq™-C0571 anti-mouse IgD
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Spark NIR™ 685 anti-mouse IgD
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TotalSeq™-B0571 anti-mouse IgD Antibody
-
Spark Violet™ 423 anti-mouse IgD
-
PE/Cyanine5 anti-mouse IgD
-
Brilliant Violet 785™ anti-mouse IgD
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