- 105 (See other available formats)
- Regulatory Status
- Other Names
- F12A2B5, 105
- Mouse IgM, κ
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- Product Citations
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The 105/A2B5 antibody recognizes a membrane protein that is expressed by chicken retinal cells, neuronal cells, ganglia, and spinal cord. In combination with other markers, this antibody can be used to differentiate between type 1 and type 2 astrocytes. The antibody recognizes polysialogangliosides and can be used to monitor glial cell development. A2B5 antigens are expressed by a variety of tumors including glioblastomas. Tumors expressing A2B5 antigen exhibit higher recurrence. The A2B5 antibody can also be used to identify progenitor cells.Product Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Host Species
- Embryonic chicken retinal cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.1,2
- Application References
- Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)
- Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)
- Russell TR, et al. 1984. Cytometry 5:539. (FC)
AB_2566394 (BioLegend Cat. No. 150703)
AB_2566395 (BioLegend Cat. No. 150704)
- Developing thymic epithelial cells, oligodendrocyte progenitors, neuroendocrine, embryonic neonatal tissue, astrocytoma, glioma, and squamous cell carcinoma.
- Cell Type
- Epithelial cells, Oligodendrocytes
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
1. Baracskay KL, et al. 2007. GLIA 55:1001.
2. Raff MC, et al. 1983. J. Neurosci. 6:1289.
3. Fredman P, et al. 1984. Arch. Biochem. Biophys. 2:661.
4. Xia CL, et al. 2003. Int. J. Oncol. 23:353.
5. Tchoghandjian A, et al. Brain Pathol. 20:211.
- Gene ID
- View information about A2B5 on UniProt.org
|Purified anti-mouse/human A2B5||105||FC,IHC-F|
|Alexa Fluor® 647 anti-mouse/human A2B5||105||FC|
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