Alexa Fluor® 647 anti-mouse Blimp-1 Antibody

Pricing & Availability
Clone
5E7 (See other available formats)
Other Names
PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5E7_AF647_Blimp-1_Antibody_FC_1_042315
Balb/c spleen cells were cultured four days in presence of LPS, then were stained with CD45R/B220 PE and IgD Brilliant Violet 421™, followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set, and staining with Blimp-1 (clone 5E7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • 5E7_AF647_Blimp-1_Antibody_FC_1_042315
    Balb/c spleen cells were cultured four days in presence of LPS, then were stained with CD45R/B220 PE and IgD Brilliant Violet 421™, followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set, and staining with Blimp-1 (clone 5E7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • 5E7_AF647_Blimp-1_Antibody_FC_2_042315
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
150003 25 µg 112€
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150004 100 µg 256€
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Description

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  2. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  3. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  4. Roy K, et al. 2019. Immunity. 50:616. PubMed
  5. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  6. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  7. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  8. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  9. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
RRID
AB_2565617 (BioLegend Cat. No. 150003)
AB_2565618 (BioLegend Cat. No. 150004)

Antigen Details

Structure
Five Kruppel-type zinc finger domains, 98 kD.
Distribution
Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.
Function
Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type
Plasma cells, T cells, NK cells, B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Nakaki F, et al. 2013. Nature 501:222.
2. Crotty S, et al. 2010. Nat. Immunol. 11:114.
3. Zhao WL, et al. 2008. Blood 111:3867.
4. Climmino L, et al. 2008. J. Immunol. 181:2338.
5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.

Gene ID
12142 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/01/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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