Alexa Fluor® 647 anti-6-His Epitope Tag Antibody

Pricing & Availability
Clone
6-His (See other available formats)
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
6-His_A647_6-His-epitope-tag_Antibody_092018
HeLa cells were transfected with (B-D) or without (A) His-tagged cytoplasmic plasmid. Cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ isotype control antibody (Negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 Anti-6-His antibody (clone 6-His) overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/35. Concentrations for (A-B) is 2.5 µg/mL (1:200 dilution), (C) is 1 µg/mL (1:500 dilution) and (D) is 0.5 µg/mL (1:1000 dilution).
  • 6-His_A647_6-His-epitope-tag_Antibody_092018
    HeLa cells were transfected with (B-D) or without (A) His-tagged cytoplasmic plasmid. Cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ isotype control antibody (Negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 Anti-6-His antibody (clone 6-His) overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/35. Concentrations for (A-B) is 2.5 µg/mL (1:200 dilution), (C) is 1 µg/mL (1:500 dilution) and (D) is 0.5 µg/mL (1:1000 dilution).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
906115 25 µg 108€
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906116 100 µg 252€
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
6xHis Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/mL (1:200-1:1,000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (ICC)
  5. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  6. Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
  7. Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
RRID
AB_2750116 (BioLegend Cat. No. 906115)
AB_2750117 (BioLegend Cat. No. 906116)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/12/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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