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Alexa Fluor® 594 anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 94 (See other available formats)
Regulatory Status
RUO
Other Names
Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SMI-94_A594_Myelin-Basic-Protein_1_020419.png
IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • SMI-94_A594_Myelin-Basic-Protein_1_020419.png
    IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • SMI-94_A594_Myelin-Basic-Protein_2_020419.png
    IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • SMI-94_A594_Myelin-Basic-Protein_3_020419.png
    IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
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836507 25 µg 123€
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836508 100 µg 259€
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.

SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Application References
  1. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
  2. Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
  3. Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
  4. McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
  5. Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
  6. Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
  7. Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
  8. Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
  9. Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
  10. Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
  11. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  12. Zhao X, et al. 2010. Neuron. 65:612. (ICC)
RRID
AB_2801176 (BioLegend Cat. No. 836507)
AB_2814559 (BioLegend Cat. No. 836508)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Cytoskeleton, plasma membrane, cytosol, and extracellular.

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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