Alexa Fluor® 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_AF488_IgG_ANtibody_FC_022014.jpg
C57BL/6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 488 (top image).
  • Poly4064_AF488_IgG_ANtibody_FC_022014.jpg
    C57BL/6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 488 (top image).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
406416 100 µg 96€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Song J,et al. 2017. Arch Virol.. 10.1007/s00705-017-3592-x. PubMed
  2. Liu J, et al. 2019. Mol Med Rep. 20:33. PubMed
  3. Procházková P, et al. 2019. Front Immunol. 10:1277. PubMed
  4. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  5. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  6. Sung P, et al. 2014. J Virol. 88:9233. PubMed
  7. Ahmed Ali A, et al. 2016. Sci Rep. 6:36650. PubMed
  8. Britschgi A, et al. 2017. Nature. 541:541-545. PubMed
  9. Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
  10. Burns JC, et al. 2020. eLife. 9:00. PubMed
  11. Singh A, et al. 2020. Mol Oncol. 1.901388889. PubMed
  12. Delbridge ARD, et al. 2021. Front Cell Neurosci. 14:592005. PubMed
  13. Bergeron S, et al. 2021. British Journal of Pharmacology. 178(7):1705-1721. PubMed
RRID
AB_2563203 (BioLegend Cat. No. 406416)

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Go To Top Version: 3    Revision Date: 02/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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