Alexa Fluor® 488 anti-mouse CD309 (VEGFR2, Flk-1) Antibody

Pricing & Availability
Clone
Avas12 (See other available formats)
Regulatory Status
RUO
Other Names
Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Avas12_AF488_030510
bEnd.3 endothelial cells stained with Avas12a1 Alexa Fluor® 488
  • Avas12_AF488_030510
    bEnd.3 endothelial cells stained with Avas12a1 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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136407 25 µg 100,00€
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136408 100 µg 220,00€
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Description

CD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine Flk1 fused to hIgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.

Application References
  1. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB)
  2. Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC-F)
Product Citations
  1. Albiero M, et al. 2014. Diabetes. 63:1353. PubMed
RRID
AB_2044064 (BioLegend Cat. No. 136407)
AB_2044065 (BioLegend Cat. No. 136408)

Antigen Details

Structure
A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
Distribution

Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle

Function
Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
Ligand/Receptor
Vascular endothelial growth factor (VEGF)
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981

Gene ID
16542 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD309 (VEGFR2, Flk-1)

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  • Alexa Fluor® 488 anti-mouse CD309 (VEGFR2, Flk-1)

  • PerCP/Cyanine5.5 anti-mouse CD309 (VEGFR2, Flk-1)

  • PE/Cyanine7 anti-mouse CD309 (VEGFR2, Flk-1)

  • TotalSeq™-A0553 anti-mouse CD309 (VEGFR2, Flk-1)

  • TotalSeq™-C0553 anti-mouse CD309 (VEGFR2, Flk-1)

  • TotalSeq™-B0553 anti-mouse CD309 (VEGFR2, Flk-1)

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