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Alexa Fluor® 488 anti-human Perforin Antibody

Pricing & Availability
Clone
B-D48 (See other available formats)
Regulatory Status
RUO
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
B-D48_A488_Perforin_Antibody_100818
Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor®488 (left), or mouse IgG1, κ Alexa Fluor®488 isotype control (right).
  • B-D48_A488_Perforin_Antibody_100818
    Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor®488 (left), or mouse IgG1, κ Alexa Fluor®488 isotype control (right).
Compare all formats See Alexa Fluor® 488 spectral data
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353319 25 tests 109€
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353320 100 tests 278€
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
RRID
AB_2783254 (BioLegend Cat. No. 353319)
AB_2783255 (BioLegend Cat. No. 353320)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis.
Cell Type
NK cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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