Product: PE anti-mouse IL-22 |
Catalog No.: 516404 |
Amlan Biswas, Instructor Boston Children's Hospital |
Nice IL-22 Detection | |||
Overall: | Product Quality: | Ease of Use: | |
This is a good antibody to detect IL-22 production by FACS. |
Experimental Design |
Application: | Intracellular cytokine assay |
Cells used: | Innate lymphoid cells |
Brief Protocol: | Lamina propria lymphocytes from Rag1-/- mice were cultured overnight with IL-23 (10 ng/ml) and stimulated with PMA plus ionomycin in the presence of monensin for the last 5h of the incubation. Cells are surface stained with Pacific Blue-CD45, APC-Cyanine7-Thy1.2, FITC-Lin and permeabilized using Fix/Perm buffer. Permeabilized cells are stained with PE-anti-mouse IL22 (1:20) for 45 min at 4 degree C. After washing the cells in staining buffer, they were analyzed by FACS. |
Results Summary: | Nice IL-22 producing CD45+ Lin- Thy1.2+ ILC population can be detected. |
Additional Notes: |
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