Product: PE anti-human CD73 (Ecto-5'-nucleotidase) |
Catalog No.: 344004 |
Isabell Baumann, Dr. UKE |
Good Antibody to Identify CD73+ T cells | |||
Overall: | Product Quality: | Ease of Use: | |
This antibody provides an excellent tool to string CD73+ T cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human peripheral blood |
Brief Protocol: | 50ul of human peripheral blood was stained with the CD73 antibody. Cells were incubated for 30 minutes at room temperature. After incubation, cells were lysed using a lysing buffer. Then samples were washed with 1ml PBS three times and cells were resuspended in 300ul FACS buffer for direct usage for flow cytometry. |
Results Summary: | This antibody clearly separates between CD73+ and CD73- T cells. |
Additional Notes: |
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