Product: ELISA MAX™ Deluxe Set Human IFN-γ

Catalog No.: 430104

sojan Abraham, Post Doc TTUHSC

Very Good ELISA Kit for Detecting Human IFN-γ
Overall:	Product Quality:	Ease of Use:
Excellent human IFN-γ ELISA kit.

Experimental Design

Application:	ELISA
Cells used:	Serum and cell culture supernatants
Brief Protocol:	1. Plates were coated with capture antibody overnight at 4 degree. 2. Plates were washed three times and blocked for 1 hr with 1X assay diluent. 3. Plates were washed three times and incubated with IFN-γ standards and unknown samples for two hours. 4. Samples were washed three times and incubated with detection antibody for 1 hr 5. Plates were washed three times and incubated with Avidin HRP for 30 min 6. Plates were washed 4 times and incubated with 100 ul substrate solution for 15 minutes. 7. After 15 minutes 100 ul stop solution was added and absorbance was measured at 450.
Results Summary:	Was able to quantify IFN-γ levels in the serum.
Additional Notes:

Data Image

Publication Status This data has been published. Pubmed

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