Product: APC/Cyanine7 anti-mouse CD3

Catalog No.: 100221

Sergey Seregin , Postdoc University of Michigan

Mouse CD3 Antibody
Overall:	Product Quality:	Ease of Use:
Reliably detects Cd3+ cells in murine splenocytes, liver lymphocytes, PBMCs.

Experimental Design

Application:	flow cytometry
Cells used:	murine splenocytes, liver lymphocytes, PBMCs
Brief Protocol:	1. Prepare single cell suspension of "cells of interest" 2. Use 2-5 million cells/tube (or per well if 96-well plate is used for staining) 3. Centrifuge cells to pellet, wash once with FACS buffer 4. Add surface antibody mix, diluted in FACS buffer (for CD3-ApcCyanine7 use 1:100 dilution) 5. Incubate 15-20 min (RT, dark) 6. Wash cells 1-2 times with FASC buffer, and either proceed to data acquisition or perform standard fixation/permeabilization protocol followed by intracellular antibody staining.
Results Summary:	Reliably detects Cd3+ cells in murine splenocytes, liver lymphocytes, PBMCs.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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