Product: FITC anti-mouse CD1d (CD1.1, Ly-38)

Catalog No.: 123508

J Lykken, Dr. Duke University

Labels CD1d on Mouse B cells
Overall:	Product Quality:	Ease of Use:
Labels CD1d on Mouse B cells, although it is not very bright.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Splenocytes
Brief Protocol:	Isolate splenocytes from mice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Stain with isotype control or CD1d FITC antibody (2.0 ug/mL in FACS buffer, 1:250 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer of 1.5% paraformaldehyde.
Results Summary:	Labels CD1d on Mouse B cells, although it is not very bright.
Additional Notes:	Works sufficiently, but CD1d conjugated to PE (Cat. # 123510) is brighter.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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