Product: PE anti-mouse IL-10

Catalog No.: 505008

J Lykken, Dr. Duke University

Optimal for the Identification of IL-10 Producing Cells - PE
Overall:	Product Quality:	Ease of Use:
Brightest IL-10 antibody to clearly identify cells that are actively producing IL-10

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Lymphocytes
Brief Protocol:	Isolate cells of interest from mice (most frequently use splenocytes, peritoneal cells, and peripheral blood cells). Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Permeabilize cells, then wash. Stain with isotype control or IL-10 PE antibody (2 ug/mL in Perm buffer, 1:100 dilution, 100 uL per million cells) for 20 minutes on ice. Fill FACS tube with Perm buffer, then allow to sit on ice for 10 minutes. Spin cells and resuspend in 100-250 uL FACS buffer of 1.5% paraformaldehyde.
Results Summary:	Clearly identifies murine IL-10+ cells on a flow cytometer as further confirmed by gene-specific reporter.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has been published. Pubmed

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