Product: APC Annexin V |
Catalog No.: 640920 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Stimulated PBMC with Annexin V APC | |||
Overall: | Product Quality: | Ease of Use: | |
Annexin V APC works well on stimulated PBMC. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Stimulated human PBMC |
Brief Protocol: | PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stimulated for 3 days with anti-CD3/CD28. Cells were washed and stained for 15 minutes at room temperature with the recommended dilution of Annexin V APC in 100uL 1x binding buffer. Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | Annexin V staining was excellent. |
Additional Notes: |
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