Product: ELISA MAX™ Standard Set Mouse TNF-α |
Catalog No.: 430902 |
Goutham Pattabiraman, Postdoctoral Fellow U Conn Health |
Mouse TNF-α ELISA MAX™ Standard is Easy to Use | |||
Overall: | Product Quality: | Ease of Use: | |
ELISA kit that provides good detection and is easy to use. |
Experimental Design |
Application: | ELISA |
Cells used: | Mouse serum |
Brief Protocol: | A plate with the capture antibody was incubated for 24 hours at 4 degrees. It was washed and blocked with assay diluent. Standards and samples were added in the plate and incubated for 2 hours. The detection antibody was washed and added for 1 hour. The enzyme Avidin-HRP was washed and added for 30 minutes. TMB solution was added and after the color developed, Stop solution was added. The plate was read with a reader with the correction wave (450/570 nm). |
Results Summary: | The ELISA kit provided a good range of detection. |
Additional Notes: |
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