Application: |
Cell culture/activation |
Cells used: |
murine T cells |
Brief Protocol: |
Plated CFSE-stained murine T cells in a 96-well U plate precoated with anti CD3 and CD28 (50uL of 1ug/mL anti-CD3 and anti-CD28 in PBS at 37*C for 2 hours, followed by 2x PBS washes) and analyze by flow cytometry 48hrs later. |
Results Summary: |
Good T cell activation was observed, as measured by CFSE dilution and up regulation of CD25. |
Additional Notes: |
The Ultra-Leaf anti-mouse CD3 and CD28 are typically used for in vitro T cell activation. As a measure of T cell activation, I used 2 different parameters- CFSE dilution, which documents that the product is functional at inducing T cell proliferation, and upregulation of CD25, which is a T cell activation marker. |
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