Product: LEGEND MAX™ Human α-Synuclein ELISA Kit

Catalog No.: 844101

Santivanez Perez , PhD student Cambridge University

Easy to Use and Highly Sensitive α-Synuclein ELISA Kit
Overall:	Product Quality:	Ease of Use:
Has a much lower detection limit compared to other colorimetric ELISA kits.

Experimental Design

Application:	Measuring α-synuclein levels in conditioned medium
Cells used:	iPSC-derived neurons
Brief Protocol:	Collected conditioned medium every 48 hrs (150 uL per 30,000 cells). Diluted samples 1:3 in kit buffer. Used white opaque plates and 100 ms integration time. Corrected levels (pg/mL) by normalising values to the protein content in conditioned medium (i.e. values if medium at 1 ug/uL).
Results Summary:	Results ranged 5x10^4-5x10^7 RLU for standards and 5x10^5-2x10^7 RLU for samples, so these can be diluted even further!
Additional Notes:	I couldn't detect α-synuclein in undiluted samples using calorimetric assays. So this works much better. As this is a very sensitive kit, it is important to dilute samples appropriately and read with low integration times to avoid saturation of wells.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews