Product: FITC anti-mouse CD3ε

Catalog No.: 100305

Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital

Staining Mouse Splenocytes with anti-CD3 FITC
Overall:	Product Quality:	Ease of Use:
Anti-CD3 FITC works well on mouse splenocytes.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	ACK-lysed mouse splenocytes were preincubated with 1ug anti-mouse FcBlock for 5 minutes and stained with a 1:100 dilution of anti-CD3 FITC for 15 minutes at room temperature in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II.
Results Summary:	CD3 staining was excellent.
Additional Notes:

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Publication Status This data has not been published.

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