Application: |
Flow cytometry |
Cells used: |
Human umbilical cord blood mononuclear cells |
Brief Protocol: |
Add 3x10^5 human umbilical cord blood mononuclear cells (CB-MNCs) in a tube. Add 50 ul diluted antibody (5 ul antibody + 45 ul PBS+2%FBS) to cells. Incubate at 4 deg C for 20-30 min. Wash 2X with PBS+2%FBS. Acquire on flow cytometer. |
Results Summary: |
Could detect human CD34+ human HSPCs in umbilical cord blood. |
Additional Notes: |
Very little background. Useful for detection of CD34+ cells pre- and post-enrichment for HSPC isolation. |
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