||Pre-working day (24h before working): Dilute your Capture antibody in the Coating buffer and add 100 microliter/well to the 96 well/plate.
Working day: Wash plate and block it for 1 hour with Assay diluent. After the blocking time wash and add your samples and standards and incubate for 2h in RT (room temperature). After 2h of incubation wash again and add the diluted Detection antibody (incubate for 1h). Then wash again and add the enzyme (diluted in Assay diluent) and incub ate for 30 min., then wash again and add the TMB Substrate solution. In the end use the Stop solution. Read the plate in a monochromator plate reader.