Product: ELISA MAX™ Standard Set Mouse IL-6
Catalog No.: 431302
Midic Ivana, PhD University of Montenegro

Mouse IL-6 ELISA MAX™ Standard
Overall: Product Quality: Ease of Use:
Confirming the inflammatory cytokines produced from macrophages under experimental conditions.
Experimental Design

Application: ELISA
Cells used: Macrophages
Brief Protocol: 1) Dilute capture antibody to the coating buffer as prescribed in the sheets and add it to the plate.
2) After overnight incubation wash + add block the plate.
3) After 1 hour wash + add standards and samples.
4) After 2 hours wash + add detection antibody.
5) After 1 hour wash + add the enzyme avidin HRP.
6) After 30 minutes wash + add TMB solution.
7) After 10-20 minutes stop the reaction using an acid.
8) Read the plate.
Results Summary: Confirming the inflammatory cytokines produced from macrophages under experimental conditions.
Additional Notes:
Data Image

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Publication Status

This data has not been published.
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