Product: Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) |
Catalog No.: 405319 |
Joyee Yao, Research Associate Ionis Pharmaceutical |
Anti-mouse IgG Has Great Sensitivity (Alexa Fluor® 488) | |||
Overall: | Product Quality: | Ease of Use: | |
Used 1:200 dilution of 0.5mg/mL Alexa Fluor® 488 secondary antibody. Signal was very strong. |
Experimental Design |
Application: | Confocal Microscopy |
Cells used: | A431 |
Brief Protocol: | Wash, Fix, Permeabilize, Block, Primary antibody (1:100-1:200), wash, secondary (1:200) wash, antifade mounting with DAPI, microscopy. |
Results Summary: | Great antibody. |
Additional Notes: | Our lab usually uses Abcam (2mg/mL) @ 1:200 dilution. |
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