Product: PE/Cyanine7 anti-mouse/rat CD29 |
Catalog No.: 102221 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Mouse Splenocytes with anti-CD29 PE/Cyanine7 | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-CD29 PE/Cyanine7 works well on mouse splenocytes. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | ACK-lysed mouse splenocytes were preincubated with 1ug anti-mouse FcBlock for 5 minutes and stained with a 1:100 dilution of anti-CD29 PE/Cyanine7 for 15 minutes at room temperature in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | CD29 staining was excellent (filled histogram), as compared to unstained cells (dotted line). |
Additional Notes: |
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