Ultra-LEAF™ Purified anti-mouse LAP (TGF-β1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Mouse Tgfb1-transduced P3U1 cells

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Neut, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone TW7-20B9 has been reported to not cross-react with human LAP.² Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.² This antibody recognizes recombinant LAP, latent TGF-ß, and pro-TGF-ß.

Additional reported applications (for relevant formats) include: Western blotting¹, immunoprecipitation¹, and neutralization². TW7-20B9 is able to neutralize certain unconventional T cell-derived forms of TGF-ß activity, but not the distinct 25 kDa free form of TGF-ß.²

Application References

(PubMed link indicates BioLegend citation)

1. Oida T, et al. 2010. PLoS ONE (FC, IP, WB)
2. Oida T, et al. 2011. PLoS ONE 6:e18365. (Neut)
3. Tu Z, et al. 2012. Invest Ophthalmol Vis Sci. 53:959. PubMed

RRID

AB_2832509 (BioLegend Cat. No. 141307)
AB_2860693 (BioLegend Cat. No. 141308)

Antigen Details

Structure

Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β

Distribution

Many cell types, highly expressed on activated Tregs and platelets

Function

TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.

Ligand/Receptor

TGF-β receptors

Cell Type

Dendritic cells, Platelets, Tregs

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction

Molecular Family

Cytokines/Chemokines, Growth Factors

Antigen References

1. Oida T, et al. 2010. PLoS
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID

21803 View all products for this Gene ID

UniProt

View information about LAP on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis