- 3/23 (See other available formats)
- Regulatory Status
- Other Names
- Bp50, TNFRSF5
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant mouse CD40 protein
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
- Bourgeois C, et al. 2002. Science 297:2060.
AB_2813996 (BioLegend Cat. No. 124627)
AB_2561490 (BioLegend Cat. No. 124628)
- 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
- Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
- CD154 on T cells
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
- Gene ID
- 21939 View all products for this Gene ID
- View information about CD40 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
|Purified anti-mouse CD40||3/23||FC,IHC-F|
|Biotin anti-mouse CD40||3/23||FC|
|FITC anti-mouse CD40||3/23||FC|
|PE anti-mouse CD40||3/23||FC|
|APC anti-mouse CD40||3/23||FC|
|Alexa Fluor® 647 anti-mouse CD40||3/23||FC|
|PE/Cyanine5 anti-mouse CD40||3/23||FC|
|PE/Cyanine7 anti-mouse CD40||3/23||FC|
|Ultra-LEAF™ Purified anti-mouse CD40||3/23||FC|
|PerCP/Cyanine5.5 anti-mouse CD40||3/23||FC|
|Pacific Blue™ anti-mouse CD40||3/23||FC|
|PE/Dazzle™ 594 anti-mouse CD40||3/23||FC|
|APC/Fire™ 750 anti-mouse CD40||3/23||FC|
|TotalSeq™-C0903 anti-mouse CD40||3/23||PG|
|TotalSeq™-A0903 anti-mouse CD40||3/23||PG|
|APC/Cyanine7 anti-mouse CD40||3/23||FC|
|TotalSeq™-B0903 anti-mouse CD40 Antibody||3/23||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD40
Biotin anti-mouse CD40
FITC anti-mouse CD40
PE anti-mouse CD40
APC anti-mouse CD40
Alexa Fluor® 647 anti-mouse CD40
PE/Cyanine5 anti-mouse CD40
PE/Cyanine7 anti-mouse CD40
Ultra-LEAF™ Purified anti-mouse CD40
PerCP/Cyanine5.5 anti-mouse CD40
Pacific Blue™ anti-mouse CD40
PE/Dazzle™ 594 anti-mouse CD40
APC/Fire™ 750 anti-mouse CD40
TotalSeq™-C0903 anti-mouse CD40
TotalSeq™-A0903 anti-mouse CD40
APC/Cyanine7 anti-mouse CD40
TotalSeq™-B0903 anti-mouse CD40 Antibody